L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator
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منابع مشابه
Bioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD
An electrochemical sensor was developed by reconstituting pyrroloquinoline quinone (PQQ), flavin adenine dinucleotide (FAD) and L-proline dehydrogenase on gold electrode. Reversible redox peaks and low redox potential were obtained from cyclic voltammograms, proved that the attached PQQ and FAD accelerated electron communication between enzyme active site and gold electrode surface. It is expec...
متن کاملPurification, characterization, and application of a novel dye-linked L-proline dehydrogenase from a hyperthermophilic archaeon, Thermococcus profundus.
The distribution of dye-linked L-amino acid dehydrogenases was investigated in several hyperthermophiles, and the activity of dye-linked L-proline dehydrogenase (dye-L-proDH, L-proline:acceptor oxidoreductase) was found in the crude extract of some Thermococcales strains. The enzyme was purified to homogeneity from a hyperthermophilic archaeon, Thermococcus profundus DSM 9503, which exhibited t...
متن کاملOxidation of hydroxy-L-proline by periodate.
Some time ago Carter and Neville reported (1) that hydroxy-n-proline yields formaldehyde on oxidation with periodate. In another laboratory, this oxidation was found to produce a considerably lower yield of formaldehyde than we had obtained.’ A further study of this reaction was accordingly undertaken. Oxidation of several new samples of hydroxy-L-proline gave lower yields of formaldehyde than ...
متن کاملConversion of C14-and cis-or trans-4-H3-L-proline to 4-hydroxy-L-proline and 4-oxo-L-proline by Streptomyces antibioticus.
26 Gerhart, J. C., and A. B. Pardee, in Synthesis and Structure of Macromolecules, Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 491. 27 Changeux, J. P., in Subunit Structure of Proteins, Brookhaven Symposia in Biology, no. 17 (1964), p. 232. 28 Antonini, E., J. Wyman, M. Brunori, J. F. Taylor, A. Rossi-Fanelli, and A. Caputo, J. Biol. Chem., 239, 907 (1964). 29 Gibson...
متن کاملMethanococcus jannaschii generates L-proline by cyclization of L-ornithine.
Cell extracts of Methanococcus jannaschii have been shown to readily convert L-ornithine to L-proline. This cyclization reaction proceeds with the loss of only the C-2 nitrogen, as has been documented for ornithine cyclodeaminase (EC 4.3.1.12). Since no gene homologous to that coding for ornithine cyclodeaminase is present in the genome of M. jannaschii, these results indicate that proline bios...
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ژورنال
عنوان ژورنال: Science and Technology of Advanced Materials
سال: 2006
ISSN: 1468-6996,1878-5514
DOI: 10.1016/j.stam.2005.12.010